生物素-16-dUTP可以通过切口平移，随机引发，3'-末端标记或在PCR过程中酶促掺入DNA中。数字'16'是dUTP和生物素之间的接头主链中的碳原子数。接头越长，生物素与抗生物素蛋白的相互作用就越有效。另一方面，接头越短，dUTP更有效地掺入DNA。生物素-16-dUTP用于在各种杂交应用中生产生物素化的DNA探针，包括Southern印迹，Northern印迹，斑点印迹，固定细胞和组织。

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