VIC 染料 qPCR 校准溶液货号67030

产品参数

Ex (nm)	526	Em (nm)	543
分子量	N/A	溶剂	Water
存储条件	在零下15度以下保存, 避免光照

产品概述

产品基本信息

产品名称：VIC 染料 qPCR 校准溶液 *10,000X*

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：NA

溶剂：水

激发波长(nm)：526

发射波长(nm)：543

产品介绍

实时热循环仪需要校准才能生成荧光信号的光谱图。由于仪器的荧光检测灵敏度可能不同，校准任何染料（如 VIC）所需的浓度将取决于所使用的设备。 AAT Bioquest VIC 染料 qPCR 校准溶液以 10,000X 浓缩液形式提供，可与多种 qPCR 仪器兼容，包括 ABI7500 Fast、QuantStudio™ 和 ViiA™ 7 系统。校准和验证应每六个月或根据需要运行一次。百萤生物是AAT Bioquest的中国代理商，为您提供优质的VIC 染料 qPCR 校准溶液 *10,000X*。

实验方案

此通用协议适用于 ABI7500 FAST qPCR 仪器。

准备 VIC 校准板：

1.将 1 μL 的 10,000X VIC 染料 qPCR 校准溶液与 10 mL 的无核酸酶水或 qPCR 缓冲液混合，制备 VIC 染料 qPCR 校准工作溶液。请注意，染料浓度必须根据仪器的校准要求进行优化。

2.在反应板的所有孔中加入 20 μL 的 VIC Dye qPCR 校准工作溶液。

3.用光学胶盖密封板

4.以 < 1500 rpm 的速度将板离心 2 分钟

5.检查板的每个孔中的液体是否位于孔的底部。如果没有，请再次以稍高的 rpm 和更长的时间将板离心

6.有关完整的校准说明，请参考您仪器的维护指南

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