品牌:AAT Bioquest
储存条件：:-15℃避光防潮
保质期：:12个月
产品规格：:200 Tests
起订:1200 Tests
供应:5200 Tests
发货:7天内
| Ex (nm) | - | Em (nm) | - |
| 分子量 | - | 溶剂 | - |
| 存储条件 | - |
Cell Meter 细胞活性检测试剂盒是美国AAT Bioquest生产的细胞活性检测试剂盒，Cell Meter检测试剂盒是一类用于检测细胞功能的系列工具，包括细胞活性、细胞毒性、细胞凋亡、细胞膜电位以及细胞周期等方面的指标。每种检测方案均能提供不同荧光颜色的检测方案。这些检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。Cell Meter细胞活力检测试剂盒 *绿色/红色双重荧光* 使用两种非荧光性指示剂：Calcein AM，用于活细胞；一种非细胞渗透性的DNA结合染料，用于细胞膜不完整的死细胞。Calcein AM是一种疏水性复合物，可以轻易地渗透进入完整的活细胞，通过酯酶的水解作用，产生强烈的荧光。
数据分析
| 图1.使用Cell Meter Cell Viability Assay Kit测量Jurkat细胞对皂苷诱导的细胞死亡的影响。 用或不用0.5％皂苷处理2×10 6个细胞/ mL的Jurkat细胞5分钟。 离心细胞，用新鲜培养基替换上清液。 将100uL未处理的细胞（A），各50μL未处理和处理的细胞（B），25uL未处理的和75uL处理的细胞（C）和100uL的0.5％皂苷处理的细胞（D）接种在 96孔黑色墙壁/透明底部聚-D-赖氨酸板。 将细胞与100μL/孔的CytoCalcein Green / Propidium Iodide染料 - 工作溶液在37℃下孵育1小时。 使用NOVOstar仪器（BMG Labtech）在底部读取模式下在Ex / Em = 490 / 525nm和540 / 650nm下测量荧光强度。 如所示（n = 6）显示活细胞和死细胞上490 / 525nm与540 / 650nm荧光强度的比率。 |
参考文献
Effect of copper nanoparticles on physico-chemical properties of chitosan and gelatin-based scaffold developed for skin tissue engineering application
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Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
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NINJ2--A novel regulator of endothelial inflammation and activation
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Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
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Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
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Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
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Inhibition of ABC transport proteins by oil sands process affected water
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公司简介
西安百萤生物科技有限公司是一家专注从事生物、化学试剂及其试剂盒的高新技术企业；主要以荧光探针，分子探针，核酸/蛋白质标记、细胞检测等技术和产品为研发核心，产品应用于基因合成、细胞检测、活体示踪，抗体标记、新药筛选等领域。
相关产品
| 产品名称 | 货号 |
| Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光* | Cat#22788 |